Select Research Articles

Cryo-EM Structure of a Human LECT2 Amyloid Fibril Reveals a Network of Polar Ladders at its Core

L.S. Richards, M.D. Flores, S. Zink, N.A. Schibrowsky, M.R. Sawaya, J.A. Rodriguez Structure (2023).

Structural consequences of sequence variation in mammalian prion β2α2 loop segments

C. Glynn, E. Hernandez, M. Gallagher-Jones, J. Miao, C.J. Sigurdson, J.A. Rodriguez Front. Neuroscience (2022).

The rippled β-sheet layer configuration—a novel supramolecular architecture based on predictions by Pauling and Corey

A. Hazari, M.R. Sawaya, N. Vlahakis, T.C. Johnstone, D. Boyer, J. Rodriguez, D.S. Eisenberg, J.A. Raskatov Chemical Science (2022).

De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

G. Treteau et al. Nature Communications (2022).

Scale-Dependent Photosalience and Topotactic Reaction of Microcrystalline Benzylidenebutyrolactone Determined by Electron Microscopy and Electron Diffraction

V.M. Hipwell, C.T. Zee, J. Rodriguez, M.A. Garcia-Garibay Crystal Growth & Design (2022).

Antibody-based inhibition of pathogenic New World Hemorrhagic Fever mammarenaviruses by steric occlusion of the human transferrin receptor 1 apical domain

S. Ferrero, M.D. Flores, C. Short, C.A. Vazquez, L.E. Clark, J. Ziegenbein, S. Zink, D. Fuentes, C. Payes, M.V. Batto, M. Collazo, C.C. García, J. Abraham, S.M. Cordo, J.A. Rodriguez, and G. Helguera, J. Virol. (2021).

Isolation and X-ray crystal structure of an electrogenerated TEMPO-N₃ charge-transfer complex

H.M. Nelson, J.C. Siu, A. Saha, D. Cascio, S.N. MacMillan, S.-B. Wu, C. Lu, J.A. Rodriguez, K.N. Houk, and S. Lin, Org. Lett. 23, 454-458 (2021).

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Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER

L.S. Richards, C. Millán, J. Miao, M.W. Martynowycz, M.R. Sawaya, T. Gonen, R.J. Borges, I. Usón and J.A. Rodriguez, Acta Cryst. D76, 703-712 (2020).

Atomic structures determined from digitally defined nanocrystalline regions

M. Gallagher-Jones, K.C. Bustillo, C. Ophus, L.S. Richards, J. Ciston, S. Lee, A.M. Minor, and J.A. Rodriguez, IUCrJ 7, 490-499 (2020).

Cryo-EM structure of a human prion fibril with a hydrophobic, protease-resistant core

C. Glynn, M.R. Sawaya, P. Ge, M. Gallagher-Jones, C.W. Short, R. Bowman, M. Apostol, Z.H. Zhou, D.S. Eisenberg, and J.A. Rodriguez, Nat. Struct. Mol. Biol. 27, 417-423 (2020).

Homochiral and racemic MicroED structures of a peptide from the ice nucleation protein InaZ

C.-T. Zee, C. Glynn, M. Gallagher-Jones, J. Miao, C.G. Santiago, D. Cascio, T. Gonen, M.R. Sawaya, and J.A. Rodriguez, IUCrJ 6(2), 197-205 (2019).

Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction

M. Gallagher-Jones, C. Ophus, K.C. Bustillo, D.R. Boyer, O. Panova, C. Glynn, C-T. Zee, J. Ciston, K.C. Mancia, A.M. Minor, and J.A. Rodriguez, Commun. Biol. 2:26, (2019).

Characterization of reactive organometallic species via MicroED

C.G. Jones, M. Asay, L. Kim, J.F. Kleinsasser, A. Saha, T.J. Fulton, K.R. Berkley, D. Cascio, A.G. Malyutin, B.M. Stoltz, V. Lavallo, J.A. Rodriguez, and H.M. Nelson, ACS Cent. Sci. 5, 1507-1513 (2019).

Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp

M. Gallagher-Jones, C. Glynn, D.R. Boyer, M.W. Martynowycz, E. Hernandez, J. Miao, C.-T. Zee, I.V. Novikova, L. Goldschmidt, H.T. McFarlane, G.F. Helguera, J.E. Evans, M.R. Sawaya, D. Cascio, D.S. Eisenberg, T. Gonen, and J.A. Rodriguez, Nat. Struct. Mol. Biol. 25, 131-134 (2018).

Analysis of global and site-specific radiation damage in cryo-EM

J. Hattne, D. Shi, C. Glynn, C.-T. Zee, M. Gallagher-Jones, M.W. Martynowycz, J.A. Rodriguez, and T. Gonen, Structure 26, 759-766 (2018).

The CryoEM method MicroED as a powerful tool for small molecule structure determination

C.G. Jones, M.W. Martynowycz, J. Hattne, T.J. Fulton, B.M. Stoltz, J.A. Rodriguez, H.M. Nelson, and T. Gonen, ACS Cent. Sci. 4, 1587–1592 (2018).

GENFIRE: A generalized Fourier iterative reconstruction algorithm for high-resolution 3D imaging

A.J. Pryor, Y. Yang, A. Rana, M. Gallagher-Jones, J. Zhou, Y.H. Lo, G. Melinte, W. Chiu, J.A. Rodriguez, and J. Miao, Sci. Rep. 7, 10409 (2017).

Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED

M.R. Sawaya, J.A. Rodriguez, D. Cascio, M.J. Collazo, D. Shi, F.E. Reyes, J. Hattne, T. Gonen, and D.S. Eisenberg, Proc. Natl. Acad. Sci.* 113, 11232-11236 (2016).

Structure of the toxic core of alpha-synuclein from invisible crystals

J.A. Rodriguez, I. Magdalena, M.R. Sawaya, D. Cascio, F. Reyes, D. Shi, E. Guenther, L. Johnson, J. Hattne, S. Sangwan, B. Nannenga, A.S. Brewster, M. Messerschmidt, S. Boutet, N.K. Sauter, T. Gonen, and D.S. Eisenberg, Nature* 525, 486-90 (2015).

Select Review Articles and Book Chapters

Electron Diffraction of 3D Molecular Crystals

A. Saha, S. Nia, and J.A. Rodriguez, Chemical Reviews, https://doi.org/10.1021/acs.chemrev.1c00879

Ab Initio Determination of Peptide Structures by MicroED

C.-T. Zee, A. Saha, M.R. Sawaya, and J.A. Rodriguez, Methods in Molecular Biology, Chapter 17, 329-348 (Springer, New York, 2021).

Taking the Measure of MicroED

J.A. Rodriguez, D.S. Eisenberg, and T. Gonen, Curr. Opin. Struct. Biol. 46, 79-86 (2017).

Toward the Atomic Structure of PrPsc

J.A. Rodriguez, L. Jiang, and D.S. Eisenberg, Prion Biology (Cold Spring Harbor Press, 2017).

High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method

J.A. Rodriguez and T. Gonen, Methods in Enzymology, 579, 369-392 (2016).