New Methods in Electron Diffraction

Solving structures by MicroED

MicroED is a new cryo-EM method used to obtain atomic resolution structures of nano-scale protein crystals. We have determined structures from the smallest protein crystals, less than 500nm on a side. Although small, these crystals can produce atomic resolution diffraction.

Phasing for MicroED

We are working on new ways to determine phases for MicroED. We currently phase MicroED data by molecular replacement, or in special cases, by direct methods.

Dynamics

When a crystal is illuminated by an electron beam, changes can occur. We are looking into how crystals change during data collection (even without being rotated) in the presence of an electron beam.

Radiation Damage

Images show a series of diffraction experiments obtained sequentially from a single crystal. We are using images like this to investigate radiation damage, the process that drives loss of high resolution diffraction from left to right.